| Issue № 1 |
Original research |
pdf-version |
Ecological issues of the nematode Rhabdias bufonis (Nematoda: Rhabdiasidae) in the steppe of the Republic of Kazakhstan
| Wakker Viktor | Ph.D., assistant professor, Pavlodar Pedagogical Institute, 140000 Pavlodar Mir st., 60, vgwacker@gmail.com |
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Keywords: Nematoda Rhabdias bufonis Rhabdiasidae moor frog steppe zone Kazakhstan |
Summary: One of the candidates for the role of a model species in the study of the biology and evolution of helminthes is the nematode R. bufonis. For this status, insufficient information has been accumulated on the nematode infection of the hosts in biotopes of different landscapes, on the long-term, seasonal and gender-age dynamics of the helminth abundance, on the peculiarities of the development cycle in natural conditions and under anthropogenic pollution. In this work, data are presented on the infection of the moor frog from 14 biotopes of the steppe zone of the Republic of Kazakhstan. Data were obtained from the autopsy of 2637 specimens of R. arvalis. Almost half of the hosts were caught at the experimental site (0.5–0.7–500 m) in the floodplain of the Irtysh River, where helminthological monitoring of R. arvalis was conducted for 14 years. It was established that the distribution of the number of R. bufonis in the populations of the moor frog belongs to the over scattered distribution. According to the level of host infection extensiveness, the abundance index, and the ratio of the actual and theoretical number of nematodes, three groups of biotopic samples were distinguished: those with high, medium, and low values of infection parameters. The infestation of the amphibian R. bufonis was determined by the presence and size of zones in the biotopes with a moistened and oxygen-enriched substrate. Part L4 of R. bufonis on the way to the lungs of the moor frogs get stuck in small vessels, enter the body cavity and accumulate. Helminthes in the body cavity grow in length, but their genitals do not develop. The nematode infestation of frogs depends on the time of sampling, the age, and to a lesser extent, the sex of the caught individuals. The highest values of the extent of invasion of the host’s lungs and body cavity and the number of helminthes in the natural conditions of the Irtysh River floodplain and in anthropogenic biotopes was observed in May and September, the lowest – in July. The minimum abundance of R. bufonis and the extent of lung invasion of R. arvalis is typical for younger age groups (body length 13.0-30.0 mm), the maximum – for older groups (body length > 36.1 mm). The host body cavity is more infected in young individuals (body length 20.1–30.0 mm). Large R. arvalis (body length > 36.1 mm) are less infected. Homing, or the proportion of R. bufonis, which successfully populate the lungs of the youngest frogs (body length ≤ 20.0 mm) is 42.3, and the largest amphibians (body length ≥ 44.1 mm) is 87.7 % In the Irtysh River floodplain and in the anthropogenic biotopes of the city of Pavlodar, the nematode twice implements a development cycle during one season. The first, spring-summer, is carried out in May – July of each current season. Post-invasive larvae of the spring generation that have penetrated the body cavity of R. arvalis remain there for 2.5–3 months. The spring generation of parasitic female nematodes lasts longer in the lungs, at that some of the individuals merge with the seasonal flow of autumn helminth generation. The development of the latter occurs in two stages. During the first one, all free-living stages of the nematode develop. It ends infecting of the host’s lungs and body cavity. Further development is interrupted by the winter dormancy of the frog and its parasites. The last part of the autumn cycle of nematode development in warm autumn (August – September) occurs within one month. The second stage of the life cycle of R. bufonis: the release of eggs by females into the lungs of frogs ends at the beginning of the next season. The second cycle of helminth development, including winter dormancy, lasts 11–13 months. R. bufonis is a species with opportunistic populations. Long-term waves of nematode abundance and infection of the lungs and body cavity of R. arvalis occur in the floodplain of the river Irtysh synchronously, with a period of 3–4 years. © Petrozavodsk State University |
Received on: 15 December 2019 Published on: 20 March 2020 | |
References
Aralhanova A. E. Features of the biology and morphology of the nematode Rhabdias bufonis from amphibians of the East Kazakhstan region, Vestnik Pavlodarskogo gosudarstvennogo universiteta. 2010. No. 1. Seriya himiko-biologicheskaya. P. 31–37.
Bakanov A. I. Quantitative rating of dominance in ecological communities. Borok: VINITI, 1987. 64 p.
Baker M. R. Seasonal population changes in Rhabdias ranae Walton, 1929 (Nematoda: Rhabdiasidae) in Rana sylvatica of Ontario, Can. J. Zool. 1979. Vol. 57. P. 179–183.
Bannikov A. G. Darevskiy I. S. Ischenko V. G. Rustamov A. K. Scherbak N. N. Manual for the identification of amphibians and repties fauna of the USSR. M.: Prosveschenie, 1977. 415 p.
Bigon M. Taunsend K. Ecology. Individuals, populations and communities. T. 1. M.: Mir, 1989. 667 p.
Boev S. N. Sokolova I. B. Panin V. Ya. Helminthes of ungulate animals of Kazakhstan. Alma-Ata: Izd-vo AN Kazahskoy SSR, 1962. T. 1. 377 p.
Breev K. A. Application of a negative binomial distribution to the study of the population ecology of parasites. Methods of parasitological research. L.: Nauka, 1972. 71 p.
Burakova A. V. Ecological-genetic features and helminthofauna of the moor frog (Rana arvalis Nilsson.) in the gradients of urbanization. Ekaterinburg, 2010. 22 c.
Combes C., Leger N., Pesson B. Variations dans le temps des populations d`Helminthes parasites de Rana temporaria L., Annales de Parasitologie (Paris). 1971. Vol. 46. No 6. P. 685–698.
Düşen S. The helminth parasites of the two bufonid toads, European Common Toad, Bufo bufo (Linnaeus, 1758) and European Green toad, Bufo (Pseudepidalea) viridis Laurenti, 1768 (Anura: Bufonidae), collected from Denizli Province, Inner-West Anatolia Region, Turkey, Helminthologia (Košice). 2011. Vol. 48. No 2. P. 101–107.
Dare O. K., Forbes M. R. Pattern of trematode nematode lungworm infections in northern leopard frogs and wood frogs from Ontario, Canada, Journal of Helmintology. 2009. Vol. 83. P. 339–343.
Dogel' V. A. The course of General Parasitology. L., 1947. 318 p.
Dubinina M. N. Ecological study of the parasite fauna of the lake frog (Rana ridibunda Pall.) in the Volga delta, Parazitologicheskiy sbornik. M.; L, 1950. T. XII. P. 340–350.
Ginecinskaya T. A. Golubeva E. B. Change of helminth fauna Rana temporaria in Peterhof Park for 50 years, Evolyuciya parazitov. Tol'yatti, 1991. P. 211–215.
Goater C. P. Experimental population dynamics of Rhabdias bufonis (Nematoda) in toads (Bufo bufo): density–dependence in the primary infection, Parasitology. 1992. Vol. 104. P. 179–187.
Goater C. P., Semlitsch R. D., Bernasconi M. V. Effects of Body Size and Parasite Infection on the Locomotory Performance of Juvenile Toads, Bufo bufo, OIKOS. 1993. Vol. 66. No 1. P. 129–136.
Goater C. P., Ward P. I. Negative Effects of Rhabdias bufonis (Nematoda) on the Growth and Survival of Toads (Bufo bufo), Oecologia. 1992. Vol. 89. P. 161–165.
Hartwich G. Über Rhabdias bufonis (Schrank, 1788) und die Abtrennung von Rhabdias dossei nov. spec. (Nematoda, Rhabdiasidae), Mitt. Zool. Museum Berlin. 1972. Bd. 48. Heft 2. S. 401–414.
Ibragimova D. V. Amphibians in the ecosystems of the city of Surgut. Novosibirsk, 2013. 16 p.
Iskakova K. I. Amphibians of Kazakhstan. Alma-Ata: Izd-vo AN Kazahskoy SSR, 1959. 145 p.
Ivanter E. V. Korosov A. V. Fundamentals of biometrics: Introduction to the statistical analysis of biological phenomena and processes. Tutorial. Petrozavodsk: Izd-vo PGU, 1992. 163 p.
Janovy J., Clopton R. E., Percival T. J. The roles of ecologic and evolutionary influences in providing structure to parasite species assemblages, J. Parasitol. 1992. Vol. 78 (4). P. 630–640.
Kennedi K. Ecological animal parasitology. M.: Mir, 1978. 230 p.
Kennedy C. R. Long-term and seasonal changes in composition and richness of intestinal helminths communities in eels Anguilla anguilla of an isolated English river, Folia Parasitologica. 1997. Vol. 44. P. 267–273.
Korytin N. S. The effect of helminth infections on the demographic parameters of the host population (on the example of common fox), Izvestiya RAN. Seriya biologicheskaya. 2012. No. 6. P. 652–657.
Korytin S. A. Space data and prediction of the quantitative and qualitative composition of populations, Chislennost' zhivotnyh i ee prognozirovanie. Kirov, 1976. P. 132–135.
Kudinova M. A. Zherihova G. V. Petrova O. E. Helminthofauna of common frogs in South Karelia, Gel'minty i ih promezhutochnye hozyaeva, Red. L. P. Shaldybin. Gor'kiy, 1985. P. 24–27.
Kuz'min S. L. Amphibians of the former USSR. M.: T-vo nauchnyh izdaniy KMK, 2012. 370 c.
Kuz'min Yu. Life cycles of rhabdiazid nematodes (Nematoda: Rhabdiasidae) from the Palaearctic, Trudy Yubileynoy konferencii Ukrainskogo nauchnogo obschestva parazitologov «Parazitologiya v Ukraine. Vchera, segodnya i zavtra». Kiev, 1995. P. 133–138.
Kuzmin J., Tkach V. Family Rhabdiasidae Railliet, 1915. 2019. URL: http://izan.kiev.ua/ppages/rhabdias/list.htm (03.08.2019).
Kuzmin Y., Junker K., Bain O. Infective larvae of Rhabdiasidae (Nematoda): comparative morphology of seven European species, Acta Parasitologica. 2014. Vol. 59 (1). P. 31–41.
Lakin G. F. Biometry. M.: Vysshaya shkola, 1990. 352 p.
Langford G. J., Janovy J. Comparative Life Cycles and Life Histories of North American Rhabdias Spp. (Nematoda: Rhabdiasidae): Lung worms from Snakes and Anurans, J. Parasitology. 2009. Vol. 95 (5). P. 1145–1155. DOI: 10.1645/GE-2044.1 https://www.researchgate.net/publication/24259593
Lees E., Bass L. Sex hormones as a possible factor influencing the level of parasitation in frogs, Nature (L.). 1960. P. 1207–1208.
Maksimov A. A. Perennial fluctuations in the number of animals, their causes and prognosis. Novosibirsk: Nauka. Sibirskoe otd-nie, 1984. 250 p.
Markov G. S. Rogoza M. L. Age dynamics of grass frog parasite fauna, Doklady AN SSSR. 1953b. T. XCIII. No. 3. P. 581–584.
Markov G. S. Rogoza M. L. Seasonal and microzonal differences in the parasitic fauna of the grass frog, Doklady AN SSSR. 1953a. T. HSIII. No. 1. P. 169–172.
Morsy K., Ali S., Abdel-Ghaffar F., El-Fayoumi H., Abdel-Haleem H. Rhabdias bufonis (Rhabdiasidae) from the lung of the African common toad, Amietophrynus regularis (Bufonidae) in Egypt. New data on the basis of light and scanning electron microscopic study. Publ: 25 Mar 2018. Peerj 6:e5328. https://doi.org/10.7717/peerj.5328
Nigon V. M., Felix M, A. History of research on C. elegans and other free-living nematodes as model organism, WormBook. 2017. 83 p. DOI: 10.1895/wormbook.1.181.1, http://www.wormbook.org
Odening K. Parasitismus. Grundfragen und Grundbegriffe. WTB Band 112. Berlin, 1974. 170 S.
Odnokurcev V. A. Sedalischev V. G. Helminth fauna of the Siberian frog of Yakutia, its sex-age and seasonal variability, Povolzhskiy ekologicheskiy zhurnal. 2008. No. 2. P. 112–119.
Pesenko Yu. A. Príncipes and methods in faunistic studies. M.: Nauka, 1982. 288 p.
Pianka E. Evolutionary ecology. M.: Mir, 1981. 400 p.
Plasota K. The effect of some ecological factors on the parasitofauna of frogs, Acta Parasit. Pol. 1969. Vol. XVI. Fasc. 6. P. 47–60.
Plohinskiy N. A. Biometry. M.: Izd-vo MGU, 1970. 359 p.
Pyatova M. V. Ravkovskaya E. A. Hvorova I. A. Lada G. A. Long-term dynamics of the species composition and abundance of helminthes of the lake frog Pelophylax ridibundus, Vestnik SPbGU. 2016. Ser. 3. Vyp. 3. P. 126–130. DOI: 10.21638/11701/spbu03.2016.321
Rezvanceva M. V. Seasonal and long-term dynamics of the number of helminths of the lake frog (Rana ridibunda) in the vicinity of Tambov, Vestnik TGU. 2009. T. 14. Vyp. 2. P. 389–393.
Savinov V. A. Some new experimental data on reservoir parasitism in nematodes, Materialy k nauchnoy konferencii Vsesoyuznogo obschestva gel'mintologov. M.: AN SSSR, 1968. Ch. 2. P. 73–75.
Schaake M. Infektionsmodus und Infektionsweg der Rhabdias bufonis Schrank (Angiostomum nigrovenosum) und die Hermaphroditischen Generation, Z. Parasitenkd. 1931. 3B. P. 518–545.
Schepina N. A. Baldanova D. R. Helminth fauna of the Mongolian toad (Bufo raddei Strauch, 1876) in Transbaikalia, Parazitologiya. 2010. T. 44. Vyp. 2. P. 153–159.
Shul'c R. S. Gvozdev E. V. Basics of General Helminthology. Helminth biology. M.: Nauka, 1972. T. 2. 516 p.
Shul'c R. S. Gvozdev E. V. Basics of General Helminthology. M.: Nauka, 1970. T. 1. 492 p.
Skryabin K. I. Shihobalova N. P. Shul'c R. S. Trichostrongylids of animals and humans. Fundamentals of Nematology. M.: Nauka, 1954. T. 3. 683 p.
Soboleva T. N. To helminth fauna of aquatic amphibians and reptiles of Kazakhstan, Ekologiya vodnyh zhivotnyh, Red. E. V. Gvozdev. Alma-Ata: AN Kaz. SSR, 1975. P. 186–192.
Sous' S. M. Perennial fluctuations in the numbers of some species of the genus Dactylogyrus (Monogenoidea, Dactylogyridae), Opyt kompleksnogo izucheniya i ispol'zovaniya Karasukskih ozer. Novosibirsk, 1982. P. 148–156.
Spieler M. Parasitologische Untersuchungen an einheimischen Moorfroschlurchen, Duisburg. Jahrbuch f. Feldherpetologie. 1994. Beiheft 2. S. 25–112.
Spieler M., Schierenberg E. On the development of the alternating free–living and parasitic generations of the nematode Rhabdias bufonis, Invertenrate Reproduction and Development. 1995. Vol. 28. No 3. P. 193–203.
Tarasovskaya N. E. Helminthofauna of terrestrial cold-blooded vertebrates of Pavlodar area and adjacent regions, Materіali H Mіzhnarodnoї naukovo-praktichnoї іnternet-konferencії «Problemi ta perspektivi rozvitku nauki na pochatku tret'ogo tisyacholіttya u kraїnah SND». Pereyaslav-Hmel'nickiy, 2013. P. 31–33.
Tarasovskaya N. E. Long-term dynamics of infection indicators of moor frogs by pulmonary nematode Rhabdias bufonis in the floodplain of the Irtysh River, Nauka segodnya: problemy i perspektivy razvitiya: Materialy mezhdunar. nauchno-prakt. konf. 30 noyabrya 2016 g. Vologda, 2016. Ch. 1. P. 157–158.
Tolokonnikov V. P. Lysenko I. O. Scientific basis of ecological parasitology. Stavropol': Izd-vo StGAU «AGRUS», 2005. P. 121–244.
Vakker V. G. Brushko Z. K. Tarasovskaya N. E. To the helminthofauna of the amphibians of Kazakhstan, Materialy Vsesoyuznogo nauchno-metodicheskogo soveschaniya zoologov pedagogicheskih vuzov SSSR, Red. P. P. Naumov. Mahachkala: Izd-vo DGU, 1990. P. 59–61.
Vakker V. G. Tarasovskaya N. E. Biology of Rhabdias bufonis (Schrank, 1788) (Rhabdiasidae) in the Middle Irtysh Region. Dep. VINITI 1988, No. 4147 – V88, ann. V RZhB 1988, 6K – 120, 17 p.
Wharton D. A. Parasites and low temperatures, Parasitology. 1999. Suppl. 119. S. 7–17.
Zaripova F. F. Fayzulin A. I. Characteristics of the functioning of the helminth community of the common frog (Pelophylax ridibundus (Pallas, 1771)) (Amphibia, Anura) in successive years in the conditions of the Trans-Urals of the Republic of Bashkortostan, Sovremennaya gerpetologiya. 2016. T. 15. Vyp. 1/2. P. 14–19.
Zhigileva O. N. Surel' O. V. Zlobina L. S. Parasitic communities of the moor frog in the south of Western Siberia, Vestnik ekologii, lesovedeniya i landshaftovedeniya. Tyumen', 2002. Vyp. 3. P. 63–68.
Zhigileva O. N. Interrelation of helminth infection and genetic diversity of animal populations. Tyumen', 2017. 327 p.
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