Klimenko O., Yakusheva N., Klimenko N., Popov A., Stepovenko V. Biopreparations as a method to biologize the agrocoenosis of the pear nursery // Principy èkologii. 2023. № 1. P. 45‒57. DOI: 10.15393/j1.art.2023.13462


Issue № 1

Original research

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Biopreparations as a method to biologize the agrocoenosis of the pear nursery

Klimenko
   Olga
D.Dc., Senior Researcher, The State Nikita Botanical Garden, 298648, the Russian Federation, the Republic of Crimea, Yalta, Nikita, Nikitsky decent, 52, olga.gnbs@mail.ru
Yakusheva
   Nina
Ph D, -, Research Institute of Agriculture of Crimea, 295043 Simferopol, Kievskaya st., 150, ninaklymenko@yandex.ru
Klimenko
   Nikolay Ivanovich
Ph D, Senior Researcher, The State Nikita Botanical Garden, 298648 the Russian Federation, the Republic of Crimea, Yalta, Nikita, Nikitsky decent, 52, klymenko.gnbs@mail.ru
Popov
   Anatoliy Ivanovich
The State Nikita Botanical Garden, 298648 the Russian Federation, the Republic of Crimea, Yalta, Nikita, Nikitsky decent, 52, sadovodstvo.koss@mail.ru
Stepovenko
   Viktor
postgraduate student, The State Nikita Botanical Garden, 298648 the Russian Federation, the Republic of Crimea, Yalta, Nikita, Nikitsky decent, 52, stepovenko14@mail.ru
Keywords:
pear nursery agrocenosis
biopreparations
plant condition and nursery productivity
fertility
rhizosphere biological activity
Summary: In the agrocenosis of a fruit nursery, a significant amount of nutrients is consumed. It requires the introduction of large doses of mineral fertilizers, which leads to environmental pollution. The use of biopreparations (BP) can increase the productivity of agrocenosis, soil fertility and its biological activity while reducing the chemical load. The aim was to study the influence of BP on the state and productivity of the agrocenosis, as well as elements of fertility and biological activity of the soil. The article presents data from field experiments on the use of biopreparations Azotobacterin-Agro (AB) and Microbiocom-Agro (MBC) in the pear nursery. BP was applied to the root system of quince rootstocks of VA 29 and KA 53 before planting; inoculation was carried out by the varieties of Tavricheskaya and Maria. The repetition of the experiment was threefold. The soil was the southern carbonate chernozem of the Crimea. In the soil (depth 0–40 cm), the content of humus, mobile forms of nutrients and the number of seven ecological and trophic groups of microorganisms were determined. It was found that BP had little effect on the survival rate of rootstocks, but they increased the survival rate and winter hardiness of the graft by 6–11 % and the growth rates of seedlings by 14–26%. The use of AB led to an increase in the yield of pear seedlings on the Tavricheskaya variety by 6–10 %, on the Maria variety by 10–21 %, depending on the rootstock. Under the influence of BP, the humus content remained stable or increased by 0.03–0.20 %. On a more intensive rootstock of KA 53, there was a depletion of the soil with nutrients, especially on the Maria variety (growing stronger). In the rhizosphere of plants grafted on VA 29, BP contributed to an increase in the content of nutrients by 5–24 % to a greater extent under the action of AB. In this case, there was an increase in the number of bacteria of the main ecological and trophic groups by 20–56 %, more significantly of autochthonous microbiota. An increase in the values of ecological and trophic indices when using AB on the Maria variety indicates an improvement in nitrogen nutrition, accumulation of humus, as well as stabilization of agrocenosis.

© Petrozavodsk State University

Received on: 17 February 2023
Published on: 11 May 2023

References

Aslantas R., Cakmakci R., Sahin F. Effect of plant growth promoting rhizobacteria on young apple tree growth fruit yield under orchard condition, Scientia Horticulture. 2007. Vol. 11, No. 4. P. 371–377.

Chaykovskaya L. A. Klyuchenko V. V. Baranskaya M. I. Ovsienko O. L. Phosphate-mobilizing bacteria in agrocenoses of Crimea. Simferopol': IT «Arial», 2018. 156 p.

Dospehov B. A. Methodology of field experiment (with the basics of statistical processing of research results). 4-e izd., pererab. i dop. M.: Kolos, 1985. 416 p.

Gridasova O. V. Verhovceva N. V. Procenko A. A. Procenko E. P. Savchenko L. A. Nevedrov N. P. Features of microbial successions in the seasonal and long-term dynamics in chernozems of the reserved meadow steppe and black fallow, Problemy agrohimii i ekologii. 2015. No. 4. P. 37–44.

Grzyb Z. S., Piotrowski W., Bielicki P., Sas Paszt L., Malusà E. Effect of different fertilizers and amendments on the growth of apple and sour cherry rootstocks in an organic nursery, Journal of Fruit and Ornamental Plant Research. 2012. Vol. 20, No. 1. P. 43–53.

Gurin A. G. Physiological aspects of mineral fertilizers' application in fruit and decorative nursery-garden, RJOAS. 2016. No. 3 (51). P. 92–98. DOI: 10.18551/rjoas.2016-03.10

Hassani M. A., Durán P., Hacquard S. Microbial interactions within the plant holobiont, Microbiome. 2018. No. 6. P. 58. DOI: 10.1186/s40168-018-0445-0.

Ivanchenko V. I. Zotikov A. Yu. The complex effect of new microbial preparations on the development of grafted grape cuttings during open stratification, Vinogradarstvo i vinodelie. 2020. T. 49. P. 157–160.

Klimenko N. I. Klimenko O. E. Popov A. I. Ecologization of apple seedlings cultivation, Byulleten' Gosudarstvennogo Nikitskogo botanicheskogo sada. 2020. Vyp. 134. P. 72–80. DOI: 10.36305/0513-1634-2020-134-72–80

Klimenko N. N. Evaluation of the effect of microbial preparations on the rootability of cuttings of grapes of the rootstock variety Shasla x Berlandieri 41 B, Problemy razvitiya APK regiona. 2019. No. 3 (39). P. 74–79.

Klimenko O. E. Klimenko N. I. Kameneva I. A. Klimenko N. N. Changes in microbiocenosis of peach seedlings rhizosphere under the influence of complex of microbial preparations, Trudy KubGAU. 2016. Vyp. 3 (60). P. 113−117.

Klymenko O., Klymenko M., Kartyzhova L., Alechenkova Z. Cultivation of sweet cherry (Cerasusavium (L.) Moench) seedlings with using of phosphate-mobilizing microorganisms, Agronomy Research (Special Issue III). 2010. No. 8. P. 633–636.

Kolomiec E. I. Innovative biotechnologies in the economy of the Republic of Belarus, Mikrobnye biotehnologii. Fundamental'nye i prikladnye aspekty: Sb. nauch. tr. 2011. T. 3. P. 7–19.

Kozlov A. V. Methods for assessing the functioning of soil microbiocenosis involved in the transformation of organic matter: Scientific and methodological manual. Nizhniy Novgorod, 2012. 64 p.

Kryzhko A. V. Smagliy N. N. Effect of Bacillus thuringiensis strains on growth and metabolic processes in Pisum sativum L. sprouts, Izvestiya vuzov. Prikladnaya himiya i biotehnologiya. 2022. T. 12, No. 4. P. 557–565. DOI: 10.21285/2227-2925-2022-12-4-557-565.

Kulagina V. I. Sungatullina L. M. Tagirov R. M. Ryazanov S. S. Hisamova A. M. Selection of microbiological indicators to perform an integrated ecological-and-biological assessment of waterlogged soils, Principy ekologii. 2019. No. 4. P. 45–56.

Kuznecova A. P. Tyschenko E. L. Trends in the development of modern nursery breeding at the present stage, Trudy KubGAU. 2015. Vyp. 4 (55). P. 124–128.

Liu M., Liu X., Cheng B, S., Ma X, L., Lyu X, T., Zhao X, F., Ju Y, L., Min Z., Fang Y, L. Selection and evaluation of phosphate-solubilizing bacteria from grapevine rhizospheres for use as biofertilizers, Spanish Journal of Agricultural Research. 2016. No. 14 (4). e1106. DOI: 10.5424/sjar/2016144-9714.

Mishustin E. N. Microorganisms and soil fertility. M.: Izd-vo AN SSSR, 1956. 342 p.

Nazari M., Smith D. L. A PGPR-produced bacteriocin for sustainable agriculture: a review of thuricin 17 characteristics and applications, Frontiers in Plant Science. 2020. Vol. 11. P. 916. DOI: 10.3389/Fpls.2020.00916.

Program and methodology of variety study of fruit, berry and nut crops, Pod red. E. N. Sedova, T. P. Ogol'covoy. Orel, 1999. 608 p.

Raheem A., Shaposhnikov A., Belimov A. A., Dodd I. C., Ali B. Auxin production by rhizobacteria was associated with improved yield of wheat (Triticum aestivum L.) under drought stress, Archives of Agronomy and Soil Science. 2018. Vol. 64. P. 574–587. DOI: 10.1080/03650340.2017.1362105.

Ryabceva T. V. Lipskaya S. L. Kamzolova O. I. The influence of biological and mineral fertilizers on the biochemical composition of fruits, leaves and agrochemical soil parameters in the apple orchard, Plodovodstvo. Samohvalovichi, 2005. T. 17, ch. 1. P. 166–171.

Sergeev D. V. Isaev R. D. The effect of mineral fertilizers on biometric indexes of pear rootstocks and one-year-old transplants in nursery, Vestnik MichGAU. 2012. No. 3. P. 102–105.

Shaposhnikov A. I. Belimov A. A. Kravchenko L. V. Vivanko D. M. Interaction of rhizosphere bacteria with plants: mechanisms of formation and factors of efficiency in associative symbiosis (review), Sel'skohozyaystvennaya biologiya. 2011. No. 3. P. 16–22.

Tepper E. Z. Shil'nikova V. K. Pereverzeva G. I. Practicum on microbiology, Pod red. V. K. Shil'nikovoy. 5-e izd., pererab. i dop. M.: Drofa, 2004. 256 p.

Tihonovich I. A. Provorov N. A. Symbioses of plants and microorganisms: molecular genetics of agricultural systems of the future. SPb.: Izd-vo Sankt-Peterburgskogo un-ta, 2009. 209 p.

Vyas P., Kaur R. Culturable stress-tolerant plant growth-promoting bacterial endophytes associated with Adhatoda vasica, Journal of Soil Science and Plant Nutrition. 2019. Vol. 19. P. 290–298. DOI: 10.1007/ s42729-019-00028-9.

и др.[Forms of microbial preparations for agriculture]. Simferopol': KGFUKC, 2007. 8 p.

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