Zaytseva T., Medvedeva N. Effect of mustard gas hydrolysis products on the development of water-bloom forming cyanobacteria // Principy èkologii. 2017. № 1. P. 70‒80. DOI: 10.15393/j1.art.2017.5942


Issue № 1

Original research

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Effect of mustard gas hydrolysis products on the development of water-bloom forming cyanobacteria

Zaytseva
   Tatyana
Ph.D., Institution of Russian Academy of Sciences Saint-Petersburg Scientific-Research Centre for Ecological Safety RAS (SRCES RAS), zaytseva.62@list.ru
Medvedeva
   Nadezhda
D.Sc., Institution of Russian Academy of Sciences Saint-Petersburg Scientific-Research Centre for Ecological Safety RAS (SRCES RAS), ngmedvedeva@gmail.com
Keywords:
mustard gas hydrolysis products
cyanobacteria
parameters of toxicity
microcystin-LR
exopolysaccharides
chlorophyll a
Summary: Mustard gas and its hydrolysis products (MGHP) belong to stable organochlorine compounds with high toxicity and broad spectrum of activity. Since the Second World War many aquatic ecosystems including the Baltic and the Adriatic Sea as well as the coastal waters of Japan, the USA, the UK, Australia have been contaminated with mustard gas due to the dumping of chemical weapon. Mustard gas and its hydrolysis products have a negative impact on aquatic life including microbiota. The aim of this work was to define the effect of MGHP on the growth, photosynthetic activity and synthesis of secondary metabolites by water-bloom forming cyanobacteria Trichormus variabilis, Aphanizomenon flos-aquae, Microcystis aeruginosa, Nodularia spumigena. Microbiological, chromatographic, spectrophotometric methods were used. The growth inhibition test with MGHP on cyanobacteria showed influence on the concentration EC50 within the range of 5.5 – 11.2 mg of organochlorine compounds (ОCC) per liter. The synthesis of chlorophyll a was also decreased. It was shown that the chlorophyll synthesis was more sensitive to MGHP than the growth of cyanobacteria. NGHP induced enhanced excretion of exopolysaccharides. Low concentration of MGHP – 0.3 mg OCC/l - promoted the growth of toxigenic cyanobacterium Microcystis aeruginosa and increased microcystin-LR concentration in the environment. enhanced excretion of such metabolites as polysaccharides and cyanotoxins has a serious negative impact on water pollution due to MGHP.

© Petrozavodsk State University

Reviewer: E. Sorokovikova
Received on: 18 November 2016
Published on: 30 March 2017

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