| Issue № 1 |
Original research |
pdf-version |
The effect of 24-epibrassinolide on growth rates, level of oxidative stress and photosynthetic pigments in little duckwood (Lemna minor L.) after exposure to heavy metals
| Bodnar Irina Sergeevna | Ph.D., Institute of Biology of Komi Scientific Centre of the Ural Branch of the Russian Academy of Sciences, 28, Kommunisticheskaya st., 167982 Syktyvkar, Komi Republic, Russia, bodnar-irina@mail.ru |
| Cheban Evgenia Vasilyevna | Institute of Biology of Komi Scientific Centre of the Ural Branch of the Russian Academy of Sciences, 28 Kommunisticheskaya st., 167982 Syktyvkar, Komi Republic, Russia, cheban.e@ib.komisc.ru |
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Keywords: Lemna minor 24-epibrassinolide heavy metals strontium cadmium copper zinc oxidative stress |
Summary: One of the mechanisms for reducing the toxic effect of excess heavy metals is the production of plant phytohormones brassinosteroids (Mandava et al., 1988). Exogenous use of 24-epibrassinolide had a positive effect on the growth and development of various plant species under the action of abiotic factors. In this study, we studied the possibility of 24-epibrassinolide to mitigate phytostress in duckweed with excessive intake of copper, cadmium, zinc, and strontium. Preliminary cultivation of a laboratory culture of little duckweed in the medium with 24-epibrassinolide allowed to increase the growth rate and reduce the share of damaged plants in experiments with zinc (6.3-79 µmol/l), copper (12.6 µmol/l) and cadmium (5, 12.6 µmol/l). The range of effectiveness in exogenous intake of brassinosteroids to reduce the toxicity of heavy metals for duckweed is as follows: Zn > Cd > Cu > Sr. Against the background of improved growth and morphometric parameters, the reduction of oxidative stress occurred only in experiments with a high concentration of strontium (1580 µmol/l). The level of malondialdehyde (MDA) in plants when combined with 24-epibrassinolide and copper ions was higher than only when adding metal (p ≤ 0.05). There was a change in the content of carotenoids and chlorophylls. The use of brassinosteroids allowed to reduce the loss of chlorophyll and carotenoids under the action of 12.6 µmol/l of cadmium and zinc (p = 0.05). However, at certain concentrations of heavy metals (for example, 12.6 µmol/l of copper), the content of photoassimilating pigments and carotenoids in plants pretreated with 24-epibrassinolide was lower than in untreated plants (p ≤ 0.05). © Petrozavodsk State University |
Received on: 24 July 2019 Published on: 14 March 2020 | |
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