| Issue № 1 |
Original research |
pdf-version |
Chromium (VI) ions induce micronuclei and nuclear abnormalities in amphibian erythrocytes
| Kryukov Vladimir Ivanovich | D.Sc., Professor, Orel State Agrarian University, 302019, Orel, General Rodin st., 69., ecogenet@mail.ru |
| Zhuchkov Sergey Aleksandrovich | Ph.D., Associate Professor, Orel State Agrarian University, 302019, Orel, General Rodin st., 69., iniic@orelsau.ru |
| Lazareva Tatyana Nikolaevna | Ph.D., Associate Professor, Orel State Agrarian University, 302019, Orel, General Rodin st., 69., iniici@orelsau.ru |
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Keywords: amphibians toads chromium (VI) mutagenicity micronuclei nuclear anomalies |
Summary: Chromium, which is one of the heavy metals, intensively pollutes the environment as a result of human activities. The toxicity, carcinogenicity and mutagenicity of chromium (VI) have been well studied in tests on fish and mammals, and to a lesser extent on birds. The genetic hazard of chromium (VI) for reptiles and amphibians is much less studied. However, amphibians are an important component of biocenoses. Therefore, the risk of exposure to chromium as an anthropogenic pollutant should be assessed for representatives of this class of vertebrates. The aim of the work was to analyze the frequency of micronuclei formation and nuclear anomalies in erythrocytes of Bufo viridis tadpoles after exposure to chromium (VI) ions. We used concentrations of 0.025, 0.050, 0.125, 0.250, 0.375 and 0.500 mg/l and the duration of exposure 6, 12, 18 and 24 hours. Experimental conditions simulated the situation of a single discharge of industrial wastewater into water bodies to final concentrations equal to 0.5, 1.0, 2.5, 5.0, 7.5 and 10 MPC of chromium (VI) ions. As a result of the experiment, it was found that chromium ions at concentrations of 0.025 and 0.050 mg/l did not cause a statistically significant increase in the total frequencies of micronuclei and nuclear anomalies in all four variants of the duration of exposure. At concentrations of 0.125 mg/l and above, the increase in the total frequencies of the analyzed anomalies was statistically significant. Consequently, even small excess of the MPC for chromium (VI) in water bodies can increase the instability of amphibian genomes. © Petrozavodsk State University |
Received on: 02 February 2023 Published on: 11 May 2023 | |
References
Luís L.G., Ferreira P., Fonte E., Oliveira M., Guilhermino L. Does the presence of microplastics influence the acute toxicity of chromium (VI) to early juveniles of the common goby (Pomatoschistus microps)? A study with juveniles from two wild estuarine populations., Aquatic Toxicology. 2015. No. 7. Vol. 164. P. 163-174.
Asad F., Mubarik M.S., Ali T., Zahoor M. K., Ashrad R, Qamer S. Effect of organic and in-organic chromium supplementation on growth performance and genotoxicity of Labeo rohita., Saudi Journal of Biological Sciences. 2019. Vol. 26. P. 1140–1145.
Blokov I.P. Environment and its protection in Russia. Changes over 25 years. M.: OMNNO «Sovet Grinpis», 2018. 422 p.
Chernogaeva G.M. Zhadanovskaya E.A. Zhuravleva L.R. Malevanov Yu.A. Environmental pollution in the regions of Russia at the beginning of the 21st century. M.: OOO «Poligraf-Plyus», 2019. 232 p.
Dabagyan N.V. Slepcova L.A. Common frog Rana temporaria L.., Ob'ekty biologii razvitiya. M.: Nauka, 1975. P. 442-462.
Fagbenro O.S., Alimba C.G., Bakare A.A. Experimental modeling of the acute toxicity and cytogenotoxic fate of composite mixtures of chromate, copper and arsenate oxides associated with CCA preservative using Clarias gariepinus (Burchell 1822)., Environ. Anal. Health. Toxicol. 2019. Vol. 34. No. 3. e2019010. URL: https://www.eaht.org/m/journal/view.php?number=832 (accessed: 4.10.2022)
Ferrier V., Gauthier L., Zoll-Moreux C., L’Haridon J. Genotoxicity Tests in Amphibians – A Review., In «Microscale Testing in Aquatic Toxicology: Advances, Techniques, and Practice» Edited by Peter G. Wells, Kenneth Lee, Christisn Blaise. –CRC Press. 2018. 720 p. (P. 507-519).
Godet F., Vasseur P. Evaluation de lagenotoxicite des effluents. Etude comparative des tests d'Ames et micronoyaux Triton. Document réalisé sous ta direction des Agences de l'Eau et du Ministère de i'Environnement Chargé d'étude : Centre des sciences de l'environnement. 1994. 184 p. URL: http://oai.eauetbiodiversite.fr/entrepotsOAI/EIA/B7655.pdf (accessed: 4.10.2022).
Gonçalves, M.W., de Campos, C.B.M., Godoy, F.R. Assessing genotoxicity and mutagenicity of three common amphibian species inhabiting agroecosystem environment., Arch. Environ. Contam. Toxicol. 2019. Vol. 77. P. 409-420. URL: https://doi.org/10.1007/s00244-019-00647-4 (accessed: 14.12.2022)
Michalová V., Galdíková M., Holečková B., Koleničová S., Schwarzbacherová V. Micronucleus assay in environmental biomonitoring., Folia Veterinaria. 2020. Vol. 64. No. 2. P. 20-28.
Mitkovska V.I., Dimitrov H.A., Chassovnikarova T.G. Chronic exposure to heavy metals induces nuclear abnormalities and micronuclei in erythrocytes of the marsh frog (Pelophylax ridibundus Pallas, 1771)., Ecologia Balkanica. 2021. Special Edition 4. –P. 97-108.
Ryabchikova T.N. Drobot G.P. Svinin A.O. Vedernikov A.A. Sidushkina M.N. Evaluation of the cytogenetic homeostasis of green frogs living in habitats with different anthropogenic pressure., Sovremennye problemy mediciny i estestvennyh nauk. 2019. No. 8. P. 156-157.
Sharma P., Singh S.P., Parakh S.K., Tong Y.W. Health hazards of hexavalent chromium (Cr (VI)) and its microbial reduction., Bioengineered. 2022. Vol. 13. No. 3. P. 4923-4938.
Udroiu I., Sgura A. , Vignoli L., Bologna M.A., D'Amen M., Salvi D., Ruzza A., Antoccia A., Tanzarella C. Micronucleus test on Triturus carnifex as a tool for environmental biomonitoring., Environmental and Molecular Mutagenesis. 2015. Vol. 56. No. 4. P.412-417.
Urbah V.Yu. Statistical analysis in biological and medical research. M.: Medicina. 1975. 295 p.
Zhuleva L.Yu. Dubinin N.P. Using the micronucleus test to assess the environmental situation in the districts of the Astrakhan region., Genetika. –1994. T. 30, No. 7. –P. 999-1004.
Zoll-Moreux C., Ferrier V. The Jaylet test (newt micronucleus test) and the micronucleus test in Xenopus: two in vivo tests on amphibia evaluation of the genotoxicity of five environmental pollutants and of five effluents., Water Research. 1999. Vol. 33, No. 10. P. 2301-2314.
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