Issue № 2 |
Original research |
pdf-version |
Morozov Nikolay Vasilyevich | Doctor of Biological Sciences, Professor, Kazan National Research Technological University, 420015, Russia, Kazan, Karl Marx st. 68, morozov_nv@mail.ru |
Ganiev Ilnur Mahmutovich | Ph. D., Kazan (Volga region) Federal University, 420008, Kazan, Paris Commune st., 9, ilnurgm-vgora@mail.ru |
Keywords: used motor oils biodegradation hydrocarbon-oxidizing microorganisms consortium spray-deficient bioreactor biotechnology scheme |
Summary: For the treatment of industrial wastewater contaminated with semi-synthetic (Shell Helix HX7 Diesel 10W-40) and mineral (I-40A, solar) oils, a column-type spray-resistant bioreactor (SRB) with three equal zones was designed and developed: 1–2 – zones of spraying, immobilization of hydrocarbon-oxidizing microorganisms (HOM) and intensive biodestruction of contaminants; 3 – zone of sedimentation and partial biooxidation of residual contaminants. Using SRB as the main apparatus developed biotechnology scheme of treatment with the following facilities: receiver clarifier waste liquid, medium-mixer, bioreactor, secondary settling tank, dispensers HOM, biogenic elements and inducing compounds, wastewater pump, pumping treated water into the medium-mixer, and the biomass of microorganisms in the dispenser. It was found that in the mode of continuous stage wastewater with used lubricants 25, 50, 75, 100 and 200 mg/dm3 in the SRB at a rate of 0.7–0.8 dm3/min (at a flow pressure up to 3.0 atm.), providing a medium temperature of 19 to 26 ºC, pH – from 6.0 to 8.0, the initial content of dissolved oxygen 1.5–3.0 mg/dm3, nutrients within 20–50 mg/dm3, inducing substances up to 35×106 M, HOM from 101×106 to 106×106 kl/dm3 and achieved purification at 92.0–98.6 % (depending on the type of contamination). The remaining amount of oils in the treated effluent is stacked in the norm of water recycling and diversion to open water sources without damaging their ecological condition. © Petrozavodsk State University |
Received on: 22 April 2021 Published on: 09 July 2022 |
Andrianova N. Zhalnina L. Roschina N. ZS. Quantitative accounting of petroleum products at gas stations. Excise taxes calculation of Russia, Ekonomika i zhizn'. 2004. No. 37. URL: http://base.garant.ru/4007974/#friends (data obrascheniya: 15.02.2020).
Chernozhukov N. I. Value of chemical composition of oils in practice of their production and operation// Himicheskiy sostav i ekspluatacionnye svoystva smazochnyh masel. M., 1957. P. 5–24.
Determinant of Bergie bacteria: V 2 t., Pod red. Dzh. Houlta i dr.; Per. s angl. pod red. akad. RAN G. A. Zavarzina. 9-e izd. M.: Mir, 1997. 429 p.
Evdokimov A. Yu. Used lubricants utilization: technologies and problems, Zaschita okruzhayuschey sredy v neftegazovom komplekse. 2005. No. 2. P. 9–11.
Ganiev I. M., Morozov N. V., Muhametzyanova A. S. Biodestruction of lubricated motor oils in sewage water with the use of pilot biological installation, J. Mater. Environ. Sci. 2019. Vol. 10. No. 6. P. 526–532.
Kasickaya L. V. Sarkisov Yu. S. Gorlenko N. P. Kopanica N. O. Kudyakov A. I. Peat resources of the Tomsk region and ways of their use in construction. Tomsk: STT, 2007. 292 p.
Lipunov I. N. Wastewater treatment in biological reactors with biofilm and activated sludge (calculation of biofilters and aerotanks): Training manual. Ekaterinburg: Ural'skiy gosudarstvennyy lesotehnicheskiy universitet, 2015. 110 p.
Morozov N. V. Ganiev I. M. Gallyamova I. N. Resource-saving biotechnology of wastewater treatment from used lubricating oils of industrial enterprises to the norms of recycling water supply, Nauchnye razrabotki: evraziyskiy region: Mezhdunarodnaya nauchnaya konferenciya teoreticheskih i prikladnyh razrabotok. M.: Infiniti, 2019. P. 191–197.
Morozov N. V. Ganiev I. M. Zinnatov F. R. Hadieva G. F. Development of biotechnology for the use of organic sorbents to activate and accelerate the biodegradation of transformer and diesel oils, Biotehnologiya: sostoyanie i perspektivy razvitiya: VIII Moskovskiy mezhdunarodnyy kongresp. M., 2015. Ch. 2. P. 336–338.
Morozov N. V., Ganiev I. M. Microbiological removal of engine oils from natural water using plant-derived sorbents, Research journal of pharmaceutical, biological and chemical sciences. 2016. Vol. 7. No. 5. P. 1728–1735.
Motor oils and ecology, Analeticheskiy portal himicheskoy promyshlennosti. Novye himicheskie tehnologii, 2006. [Elektronnyy resurs]. URL: http://newchemistry.ru/letter.php?n_id=329 (data obrascheniya: 10.02.2020).
Muratova A. Yu. Pleshakova E. V. Microbiological purification in environmental protection from oil pollution, Problemy izucheniya biosfery: Tezisy dokladov Vserossiyskoy konferencii. Saratov, 1996. P. 107–108.
Petrov A. A. Hydrocarbons of oil. M.: Nauka, 1984. 263 p.
Potashnikov Yu. M. Utilization of production and consumption wastes (textbook). Tver': Izd-vo TGTU, 2004. 107 p.
Quantitative chemical analysis of water. The technique of measuring the chemical oxygen consumption in samples of natural and treated wastewater by the titrimetric method. M.: Izd-vo standartov, 1997. 16 p.
Quantitative chemical analysis of water. The technique of measuring the mass concentration of sulfate ions in samples of natural and waste water by turbidimetric method. M.: Izd-vo standartov, 2000. 14 p.
Quantitative chemical analysis of waters. Method (method) of measuring the mass concentration of petroleum products in wastewater samples by infrared spectrophotometry with the use of concentrators series KN. M.: Izd-vo standartov, 2012. 22 p.
Quantitative chemical analysis of waters. Methods for measuring the mass concentration of ammonium ions in drinking, surface (including marine) and waste water by photometric method with Nessler reagent. M.: Izd-vo standartov, 2010. 26 p.
Quantitative chemical analysis of waters. Methods of measuring the mass concentration of nitrates ions in drinking, surface and waste water by the photometric method with salicylic acid. M.: Izd-vo standartov, 1995. 18 p.
Quantitative chemical analysis of waters. Methods of measuring the mass concentration of nitrite ions in drinking, surface and waste water by the photometric method with Grisse reagent. M.: Izd-vo standartov, 1995. 22 p.
The technique of mass concentration measurements of dissolved oxygen in samples of natural and treated wastewater by iodometric method. M.: Izd-vo standartov, 1997. 20 p.
Timergazina I. F. Perehodova L. S. To the problem of biological oxidation of oil and oil products by hydrocarbon-oxidizing microorganisms (in Russian), Neftegazovaya geologiya. Teoriya i praktika. 2012. T. 7. No. 1. P. 15.
Zernov Yu. P. Use of restriction analysis of the amplified gene 16S RNA for the indexation of microorganisms on the example of bacterial producers of thermolabile alkaline fascatase, Biotehnologiya. 2005. No.6. P. 3–11.
Zhukova O. V. Morozov N. V. Interaction of microorganisms with solid surfaces - sorbents during removal of local oil pollution, Vestnik TGGPU. 2010. No. 3 (21). P. 99–106.