| Issue № 1 |
Original research |
pdf-version |
Indicators of the leukocite system of the blood of the tesselated water snake Natrix tessellata of the Northern and Eastern Pre-Caspia
| Romanova Elena Borisovna | D.Sc., N.I.Lobachevsky National Research University of Nizhny Novgorod, 23 Gagarin Av., Nizhny Novgorod 603950, Russia, romanova@ibbm.unn.ru |
| Solomaykin Evgeny Igoreviсh | N.I.Lobachevsky National Research University of Nizhny Novgorod, 23 Gagarin Av., Nizhny Novgorod 603950, Russia, e7v4gen5iy@yandex.ru |
| Bakiev Andrey Gennadyevich | Ph.D., assistent professor, Samara Federal Research Center RAS, Institute of Ecology of the Volga River Basin of RAS, Russia, 10 Komzin st., Togliatti 445003, Russia, herpetology@list.ru |
| Gorelov Roman Andreevich | Ph.D., Samara Federal Research Center RAS, Institute of Ecology of the Volga River Basin of RAS, Russia, 10 Komzin st., Togliatti 445003, Russia, gorelov.roman@mail.ru |
| Klenina Anastasia Aleksandrovna | Ph.D., Samara Federal Research Center RAS, Institute of Ecology of the Volga River Basin of RAS, Russia, 10 Komzin st., Togliatti 445003, Russia, colubrida@yandex.ru |
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Keywords: tesselated water snake Natrix tessellata WBC (white blood cells) leukocyte indexes peripheral blood |
Summary: Snakes have a well-developed hematopoietic and immune system, demonstrate responses to the full range of environmental factors and are a unique object for environmental research. The issues of assessing the immune status of snakes under the influence of anthropogenic press in different biotopic environmental conditions remains poorly understood. Therefore, it is particularly relevant to search for population markers that allow us to assess the work of the effector mechanisms of the immune system determining the process of forming adaptive responses of the body. The aim of the work was a comparative assessment of the leukocyte blood system of the tesselated water snake Natrix tessellata (Laurenti, 1768) of the Northern and Eastern Pre-Caspian. The lymphocyte-granulocyte composition of peripheral blood of the water snake Natrix tessellata was characterized by a predominance of agranulocytes (70–75 %), the proportion of granulocytes was 25–30 %. The redistribution of the ratio of granulocytes to agranulocytes was established in the blood of individuals of the studied populations from different biotopes. The content of heterophiles, monocytes was increased and content of lymphocytes was reduced in individuals of the Eastern Pre-Caspian compared with individuals of the Northern Pre-Caspian. Intersex differences were observed in the content of azurophils, the proportion of which in the blood of females of the water snake from the Northern Pre-Caspian was higher compared to males. Integral indices (ISGE, ILG, ISGL) revealed the same type of variability in the parameters of the blood leukocyte system of females and males living in the areas of the Caspian lowland. The revealed differences in the parameters of the white blood cell system of water snakes may be related to their seasonal ontogenetic activity. The non-specific protective system of the blood of snakes responds faster and more effectively to a wide range of pathogenic antigens of the environment, compared with adaptive responses. Consequently, during the mating period, it is the innate defense system, characterized by higher development in ectothermic animals that provided maximum protection and stable functioning of the body. © Petrozavodsk State University |
Received on: 18 July 2020 Published on: 28 March 2021 | |
References
Arican H., Cicek K. Morphology of peripheral blood cells from various species of Turkish herpetofauna, Acta Herpetologica. 2010. Vol. 5, No 2. P. 179–198.
Bakiev A. G. Malenev A. L. Zayceva O. V. Shurshina I. V. Snakes of Samara region. Tol'yatti: OOO «Kassandra», 2009. 170 p.
Bakiev A., Kirillov A., Mebert K. Diet and Parasitic Helminths of Dice Snakes from the Volga Basin, Russia, The Dice Snake, Natrix tessellata: Biology, Distribution and Conservation of a Palaearctic Species, Mertensiella. 2011. No. 18. P. 325–329.
Campbell T. W. Clinical pathology of reptiles, Reptile medicine and surgery. 2nd edition. St. Louis (MO): Saunders Publishing, 2006. P. 453–470.
Chuguevskaya N. M. Snakes of the genus Natrix (Serpentes, Colubridae, Natrix) of the Volga Basin: Ecology and Conservation: Avtoref. dip. … kand. biol. nauk. Tol'yatti, 2005. 20 p.
Coico R. Immunology. A Short Course. Hoboken; NJ: Wiley-Liss Publications, 2003. 237 p.
Cooper E. L., Klempau A. E., Zapata A. G. Reptilian Immunity, Biol. Reptilia. New York, 1985. Vol. 14. P. 601–636.
Davis A. K., Maney D. L., Maerz J. C. The use of leukocyte profiles to measure stress in vertebrates: a review for ecologists, Functional Ecology. 2008. Vol. 22. P. 760–767.
Hayrutdinov I. Z. Sokolina F. M. Characterization of reptile blood and its relationship with environmental conditions. Kazan': Kazanskiy universitet, 2010. 44 p.
International Recommendations for Biomedical Research Using Animals. 2012. URL: https://grants.nih.gov/grants/olaw/guiding_principles_2012.pdf
Klenina A. A. Bakiev A. G. Volume of eggs in the clutches of grass snake Natrix natrix and dice snake N. tessellata: error correction, Principy ekologii. 2015. T. 4, No. 4. C. 11–21.
Klenina A. A. Gordeev D. A. Prilipko S. K. Diet of snakes of the genus Natrix in Volgograd region, Izvestiya Samarskogo nauchnogo centra Rossiyskoy akademii nauk. 2015. T. 17, No. 4 (4). P. 718–720.
Kobolkuti L., Cadar D., Czirjak G., Niculae M. et al. The Effects of Environment and Physiological Cyclicity on the Immune System of Viperinae, The Scientific World Journal. 2012. Vol. 4. DOI: 10.1100/2012/574867
Lisicic D., Dikic D., Benkovic V., Knezevic A. H. et al. Biochemical and hematological profiles of a wild population of the nose-horned viper Vipera ammodytes (serpentes: Viperidae) during autumn, with a morphological assessment of blood cells, Zoological studies. 2013. Vol. 52, No. 1. P. 11–20.
Litvinov N., Bakiev A., Mebert K. Thermobiology and Microclimate of the Dice Snake at its Northern Range Limit in Russia, The Dice Snake, Natrix tessellata: Biology, Distribution and Conservation of a Palaearctic Species, Mertensiella. 2011. No. 18. P. 330–335.
Pavlov A. V. Yusupov R. H. Blood system, Gadyuki (Reptilia: Serpentes: Viperidae: Vipera) Volzhskogo basseyna. Ch. 1. Tol'yatti: Kassandra, 2015. P. 137–155.
Pavlov A. V. Key points of reptiles hematology: peculiarities of estimation of the leukocyte part of the blood, Izvestiya vysshih uchebnyh zavedeniy. Povolzhskiy region. Estestvennye nauki. 2019. No. 1 (25). P. 138–152. DOI: 10.21685/2307-9150-2019-1-14.
Rebrova O. Yu. Statistical analysis of medical data. M.: MediaSfera, 2006. 312 p.
Romanova E. B. Nikolaev V. Yu. Bakiev A. G. Klenina A. A. Features of leukocyte blood composition of females of grass snake (Natrix natrix) and dice snake (N. tessellata) in the Samara region, Sovremennaya gerpetologiya. 2015. T. 15. Vyp. 1/2. P. 69–76.
Romanova E. B. Solomaykin E. I. Bakiev A. G. Gorelov R. A. Klenina A. A. Immunohematological indicators of non-poisonous and poisonous snakes on the territories of the Volga river basin with different antropogenic transformation, Izvestiya Samarskogo NC RAN. 2017. T. 19, No. 2. P. 54–61.
Romeys B. Microscopic technique, Per. s nem. prof. V. Ya. Aleksandrova i Z. I. Kryukova; Pod red. I. I. Sokolova. M.: Izd-vo inostr. lit., 1954. 719 p.
Salakij C., Salakij J., Chanhome L. Comparative hematology, morphology and ultrastructure of blood cells in Monocellate cobra (Naja kaouthia), Siamese spitting cobra (Naja siamensis) and Golden spitting cobra (Naja sumatrana), Kasetsart J. (Nat. Sci.). 2002. Vol. 36. P. 291–300.
Tabachishin V. G. Tabachishina I. E. Dissemination and particularities of ecology of the grass snake (Natrix natrix) on the north of the lower Volga region, Povolzhskiy ekologicheskiy zhurnal. 2002. No. 2. P. 179–183.
Vasil'ev D. B. Veterinary Herpetology. M.: Akvarium, 2016. 392 p.
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