Kulagina V., Sungatullina L., Tagirov R., Ryazanov S., Khisamova A. Selection of microbiological indicators to perform an integrated ecological-and-biological assessment of waterlogged soils // Principy èkologii. 2019. № 4. P. 45‒56. DOI: 10.15393/j1.art.2019.9262

Issue № 4

Original research


Selection of microbiological indicators to perform an integrated ecological-and-biological assessment of waterlogged soils

   Valentina Ivanovna
Ph.D., Research Institute for Problems of Ecology and Mineral Wealth Use of Tatarstan Academy of Sciences, viksoil@mail.ru
   Lutsia Mansurovna
Research Institute for Problems of Ecology and Mineral Wealth Use of Tatarstan Academy of Sciences, sunlyc@yandex.ru
   Ramis Marselevich
Research Institute for Problems of Ecology and Mineral Wealth Use of Tatarstan Academy of Sciences, ramis.tagirov@yandex.ru
   Stanislav Sergeevich
Research Institute for Problems of Ecology and Mineral Wealth Use of Tatarstan Academy of Sciences, erydit@yandex.ru
   Alina Maratovna
Research Institute for Problems of Ecology and Mineral Wealth Use of Tatarstan Academy of Sciences, his.alina94@mail.ru
soil microorganisms
integrated indicator
waterlogging of soil
ecological-and-biological state
biological activity
Summary: Integrated ecological and biological indicator provides wide possibilities for assessment of anthropogenic impact on soils and bio-monitoring. Each type of anthropogenic impact is characterized by changes in certain sets of biological soil parameters. To calculate the integrated indicator, one should select the parameters that change statistically significantly under the influence of a particular adverse factor. The article presents the results of work on the identification of microbiological parameters most sensitive to water logging in soil. The research was performed using samples of leached chernozem and alluvial sod soils which were 3.5 months in controlled conditions of the model experiment on the effect of water logging on soils. Soil samples were kept in plastic containers in conditions of water logging, at optimal moisture content, with and without addition of sucrose solution. At the end of the experiment, the population of five ecological trophic groups of microorganisms was determined by seeding on solid nutrient media. The determination was carried out in triplicate. The statistical significance of the difference in the population of microorganism groups between flooded soils and soils with optimal humidity was determined. It was shown that water logging led to a reduction in population of the most studied microorganisms. It was established that according to their sensitivity to water logging and the processes of gleization, the microorganisms can be arranged in the following series in descending order: microscopic fungi, actinomycetes, amylolyticus, ammonifiers, and spores. The spore microorganisms are most resistant to water logging. Statistical analysis did not reveal a relationship between their population in alluvial soils and ox-redox potential. It was shown that the integrated indicator calculated taking into account the population of only the three most sensitive groups of microorganisms has a higher correlation coefficient with the soil ox-redox potential than that calculated taking into account all five groups.

© Petrozavodsk State University

Received on: 27 June 2019
Published on: 19 December 2019



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