Erofeeva E., Rechkin A., Savinov A. Seed germination and seedling state of wheat Triticum aestivum when exposed to Azotobacter chroococcum cell suspension // Principy èkologii. 2019. № 2. P. 4‒11. DOI: 10.15393/j1.art.2019.8405


Issue № 2

Original research

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Seed germination and seedling state of wheat Triticum aestivum when exposed to Azotobacter chroococcum cell suspension

Erofeeva
   Elena Alexandrovna
D.Sc., Lobachevski State University of Nizhny Novgorod, ele77785674@yandex.ru
Rechkin
   Alexander Ivanovich
Ph. D., Lobachevski State University of Nizhny Novgorod, re-ka@mail
Savinov
   Alexander Borisovich
Ph. D., Lobachevski State University of Nizhny Novgorod, sabcor@mail.ru
Keywords:
Azotobacter
Triticum aestivum
pigment
lipid peroxidation
growth
seed germination
Summary: To date, the ability of various Azotobacter strains to enter into symbiosis with the wheat and regulate its development has not been studied enough. In this regard, we assessed the capacity of strain No. 4 of Azotobacter chroococcum isolated from the soil of agricultural land in Nizhny Novgorod region (Russia) to influence the seed germination and the state of wheat seedlings (Triticum aestivum L.) when various quantities of cells were introduced into the nutrient solution (from 109 cells/ml (A) to A/256) in the first day of the experiment. It was stated that all cell concentrations except for the smallest one caused reduced seed germination. Low concentrations (А/256-А/64) did not affect the wet biomass of seedlings root; medium ones А/32-А/16 increased it. At high concentrations А/8 the effect disappeared, and the highest concentrations А/4-А again increased this parameter. As for the wet shoot biomass, a stimulating effect was caused only by the concentrations A/2 and A/4. Chlorophyll and carotenoids content, as well as the intensity of lipid peroxidation did not change under the action of A. chroococcum. Thus, the studied strain of A. chroococcum is capable of regulating the germination of seeds and the growth of the root system and shoots of T. aestivum.

© Petrozavodsk State University

Received on: 07 November 2018
Published on: 02 July 2019

References

 Erofeeva E. A. Hormesis and paradoxical effects of wheat seedling (Triticum aestivum L.) parameters upon exposure to different pollutants in a wide range of doses, Dose Response. 2014. Vol. 12. No 1. P. 121–135.

Feoktistova N. V. Mardanova A. M. Hadieva G. F. Sharipova M. R. Rhizosphere bacteria, Uchenye zapiski Kazanskogo universiteta. Ser. Estestvennye nauki. 2016. T. 158. Kn. 2. P. 207–224.

Howard J. B., Rees D. C. How many metals does it take to fix N2? A mechanistic overview of biological nitrogen fixation, Proceedings of the National Academy of Sciences of the United States of America. 2006. Vol. 103. No 46. P. 17088–17093.

Kamyshnikov V. S. Reference book on clinical and biochemical laboratory diagnostics. Minsk: Belarus', 2002. T. 2. 495 p.

Kirichenko E. Titova L. Koc' S. The bacterization effectiveness of spring wheat seeds with a new strain of Аzotobacter chroococcum T76, Stiinta Agricola. 2010. No. 1. P. 21–24.

Kirichenko E. V. Koc' S. Ya. The use of Azotobacter chroococcum to create complex biological preparations, Biotehnologiya. 2011. T. 4. No. 3. P. 74–81.

Kirichenko E. V. Biological activity of spring wheat rhizosphere soil in association with Azotobacter chroococcum T79 bacteria modified with N-acetyl-D-glucosamine, Mikrobiologiya i biotehnologiya. 2016. No. 3. P. 30–42.

Nefed'eva E. E. Belopuhov S. L. Verhoturov V. V. Lysak V. I. The role of phytohormones in the regulation of seed germination, Izvestiya vuzov. Prikladnaya biohimiya i biotehnologiya. 2013. No. 1. P. 61–65.

Salabrese E. J., Blain R. B. The occurrence of hormetic dose responses in the toxicological literature, the hormesis database: an overview, Toxicology and Applied Pharmacology. 2005. Vol. 202. P. 1451–1474.

Shlyk A. A. Determination of chlorophylls and carotenoids in green leaf extracts, Biohimicheskie metody v fiziologii rasteniy. M.: Nauka, 1971. P. 154–170.

Wani S. A., Chand S., Ali T. Potential use of Azotobacter chroococcum in crop production, Current Agriculture Research Journal. 2013. Vol. 1. No 1. P. 35–38.

Weyers J. D., Paterson N. W. Plant hormones and the control of physiological processes, New Phytol. 2001. Vol. 152. P. 375–407.

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