Morozova T., Semyonov S. Evaluation of the possibility of using brewer’s spent grain for the fermentation of lignocellulosic hydrolysates to biobutanol // Principy èkologii. 2017. № 2. P. 38‒46. DOI: 10.15393/

Issue № 2

Original research


Evaluation of the possibility of using brewer’s spent grain for the fermentation of lignocellulosic hydrolysates to biobutanol

   Tatyana Sergeevna
National Research Tomsk State University,
   Sergey Yuryevich
Ph.D., National Research Tomsk State University,
Brewer’s grain
lignocellulosic hydrolysate
Clostridium acetobutylicum АТСС 824
growth substances
amino acids
Summary: The paper deals with the investigation of the possible using of brewer’s grain as a source of growth substabces in acetone-butanol fermentation of lignocellulosic hydrolysates in order to reduce the cost of biobutanol production and to utilize the brewery waste. The fermentation of glucose was carried out at different concentrations of the brewer’s grain by Clostridium acetobutylicum ATCC 824. In the experiments on fermentation of the lignocellulosic hydrolysates an enzymatic hydrolysate of miscanthus cellulose containing 34.8 g/l glucose and 15.6 g/l xylose was used as a source of reducing substances. The sterilization of the medium was carried out at 0.5 KPa for 20 minutes. The sterilization of the growth and reducing substances sources was conducted separately to prevent caramelization of products and melanoidins. For inoculation the spores of 3% (vol/vol) C. acetobutylicum ATCC 824 were transferred to a fresh medium. The strain was grown at 37 °С under anaerobic conditions. In a series of experiments on the evaluation of the influence of the brewer’s grain on the fermentability of carbohydrates by the strain of C. acetobutylicum АТСС 824, limiting and inhibitive concentrations of brewer’s grain were determined in the medium, which were 2 and 20 % vol., respectively. The optimal amount of the brewer’s grain was about 6 % vol. At the optimal concentration of the brewer’s grain the fermentation of lignocellulosic hydrolysates occured in all replicates. It was characterized by intensive gas and foam formation that corresponds to the data in literature. After 79-88 h of fermentation of miscanthus cellulose hydrolysate the product yield amounted 10.14±0.87 g/L butanol, 02.48±0.53 acetone, 01.02±0.42 g/L ethanol. It was found that at an optimum concentration both the fresh and sour brewer’s grain can be used in the fermentation. After the acetone-butanol fermentation the brewer’s grain can be used as a food for farm animals. The obtained results indicate the possibility of using the brewer’s grain as a source of growth substances for the fermentation of lignocellulosic hydrolysates. The proposed method of using the brewer’s grains in acetone-butanol fermentation may reduce the cost of biobutanol and also solve the problem of disposing some waste from the brewing industry.

© Petrozavodsk State University

Received on: 23 December 2016
Published on: 04 July 2017



Annous B. A., Blaschek H. P. Regulation and Localization of amylolytic enzymes in Clostridium acetobutylicum ATCC 824, Applied and environmental microbiology. 1990. Vol. 56 (8). P. 2559–2561.

Croux Ch., Canard B., Goma G. Autolysis of Clostridium acetobutylicum ATCC, Journal of General Microbiology. 1992. Vol. 138. P. 861–869.

Logotkin I. S. Technologies of Acetone-Butanol Production. M.: Pischepromizdat, 1958. 254 p.

Morone A., Pandey R. A. Lignocellulosic biobutanol production: Gridlocks and potential remedies, Renewable and Sustainable Energy Reviews. 2014. Vol. 37. P. 21–35.

Morozova T. S., Semyonov, S. Y. Biological detoxification of lignocellulosic hydrolysates for improved biobutanol production, Key Engineering Materials. 2016. Vol. 683. P. 525–530.

Nanda S., Dalai A. K., Kozinski J. A. Butanol and ethanol production from lignocellulosic feedstock: biomass pretreatment and bioconversion, Energy Science & Engineering. 2014. Vol. 2 (3). P. 138–148.

Ounine K., Petitdemange H., Raval G., Gay R. Acetone-butanol production from pentoses by Clostridium acetobutylicum, Biotechnology Letters. 1983. Vol. 5 (9). P. 605–610.

Petrov S. M. Filatov S. L. Pivnova E. P. Shibanov V. M. To the Question of the Methods of Disposal of Brewer's Grains, Pivo i Napitki. 2014. No. 6. P. 32–37.

Procentese A., Raganatia F., Olivieria G., Russo M. E., Feld M., Marzocchella A. Renewable feedstocks for biobutanol production by fermentation, New Biotechnology. 2016. 29 October. P 1–6.

Qureshi N., Ezeji T. C. Isolation Butanol, «a superior biofuel» production from agricultural residues (renewable biomass): recent progress in technology, Biofuels. Bioprod. Bioref. 2008. Vol. 2. P. 319–330.

Roth J., Tippkӧtter N. Evaluation of lignocellulosic material for butanol production using enzymatic hydrolysate medium, Cellulose Chem. and Technol. 2016. Vol. 50 (3–4). P. 405–410.

Rudenko E. Yu. Modern Trends in Processing of the Main By-products of Brewing, Pivo i Napitki. 2007. No. 2. P. 66–68.

The Environmental doctrine of the Russian Federation: Decree of the RF Government dated 31 Aug. 2002 No. 1225-R, SPS Konsul'tantPlyup.

Xiros Ch., Christakopoulos P. Enhanced ethanol production from brewer's spent grain by a Fusarium oxysporum consolidated system, Biotechnology for Biofuels. 2009. Vol. 2. P. 4–10.

Yarovenko V. L. Nahmanovich B. M. Scheblykin N. P. Senkevich V. V. Continuous Fermentation in Acetone-butanol Production. Nal'chik: Kabardino-balkarskoe knizhnoe izdatel'stvo, 1963. 327 p.

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