Sladkova S., Kholodkevich S., Safronova D., Borisov R. Cardiac activity of crayfish Cherax quadricarinatus (von Martens 1868) in different physiological states // Principy èkologii. 2017. № 3. P. 40‒53. DOI: 10.15393/

Issue № 3

Analytical review


Cardiac activity of crayfish Cherax quadricarinatus (von Martens 1868) in different physiological states

Scientific Research Centre for Ecological Safety, Russian Academy of Sciences; Saint-Petersburg State University,
Scientific Research Centre for Ecological Safety, Russian Academy of Sciences; Saint-Petersburg State University,
Saint-Petersburg State University,
Russian Federal Research Institute of Fisheries and Oceanography,
crayfish Cherax quadricarinatus
cardiac activity
circadian rhythm
Summary: Diurnal cardiac rhythm of crayfish Cherax quadricarinatus was studied. Crayfish were contained in laboratory conditions with the following illumination mode: 12 hours of light, 12 hours of darkness. Real-time heart rate was recorded non-invasively for several months using the original fiber-optic method. It was stated that after a certain period of acclimation a pronounced diurnal rhythm was established in crayfish. It changes during the pre-molting stage of the molting cycle and disappears completely within 3-5 days immediately preceding molting. It was shown that changes in the chronotropic characteristics of cardiac activity such as cardiac rhythm found in these experimental conditions could serve as reliable criteria for the assessment of the functional state of the crayfish, which is used as a test-organism for the assessment of surface waters ecological state.

© Petrozavodsk State University

Reviewer: A. Dvoretsky
Reviewer: N. Y. Koryagina
Received on: 21 March 2017
Published on: 28 October 2017


Ahearn G. A., Mandal P. K., Mandal A. Calcium regulation in crustaceans during the molt cycle: a review and update, Comp. Biochem. Physiol. A. Mol. Integr. Physiol. 2004. Vol. 137. P. 247–257.

Aiken D. E., Waddy S. L. The growth-process in crayfish, Rev. Aquat. Sci. 1992. Vol. 6. P. 335–381.

Aiken D. E., Waddy S. L. Winter temperature and spring photoperiod requirements for spawning in the American lobster (Homarus americanus), J. Shellfish Res. 1990. Vol. 9. P. 41–43.

Armitage K. B., Buikema A. L., Willems N. J. The effect of photoperiod on organic constituents and molting of the crayfish Orconectes nais (Faxon), Comp. Biochem. Physiol. 1973. Vol. 44(A). P. 431–456.

Baevskiy R. M. Berseneva A. P. Evaluation of adaptive abilities of an organism and risk of diseases development. M.: Medicina, 1997. 235 p.

Bamber S. D., Depledge M. H. Responses of shore crabs to physiological challenges following exposure to selected environmental contaminants, Aquatic Toxicology. 1997. Vol. 40. P. 79–92.

Barki A., Levi T., Hulata G., Karplus I. Annual cycle of spawning and molting in the red-claw crayfish, Cherax quadricarinatus, under laboratory conditions, Aquaculture. 1997. Vol. 157. P. 239–249.

Bojsen B. N., Witthofft H., Styrishave B. In situ studies on heart rate and locomotor activity in the noble freshwater crayfish, Astacus astacus (L.) in relation to natural fluctuation in temperature and light intensity, Freshwater Biology. 1998. Vol. 39. P. 455–465.

Carreño-León D., Racotta-Dimitrov I., Casillas-Hernández R., Monge-Quevedo A., Ocampo-Victoria L., Naranjo-Páramo J., Villarreal H. Growth, metabolic and physiological response of juvenile Cherax quadricarinatus fed different available nutritional substrates, J. Aquac. Res. Development. 2014. Vol. 5. No. 2. Doi:10.4172/2155-9546.1000220.

Cherkashina N. Ya. Dynamics of Pontastacus and Caspiastacus crayfish populations (Crustacea, Decapoda, Astacidae) and methods for increasing their abundance. M.: Nacrybresurs, 2002. 256 p.

Cukersis Ya. M. Biology of the crayfish Astacus astacus. Vil'nyus: Milyatis, 1970. 204 p.

Depledge M. H. Disruption of circulatory and respiratory action in shore crabs Carcinus maenas (L.) exposed to heavy metal pollution, Comp. Biochem. Physiol. 1984. Vol. 78. P. 445–459.

Drach P. Mue et cycle d’intermue chez les Crustacés décapodes. Ann. Inst. Océanogr. Paris (Monaco), 1939. 19. pp. 103–391.

Dubé P., Portelance B. Temperature and photoperiod effects on ovarian maturation and egg laying of the crayfish Orconectes limosus, Aquaculture. 1992. Vol. 102. P. 161–168.

Fedotov V. P. Holodkevich S. V. Strochilo A. G. Peculiarities of cardiac activity in the crayfish Astacus astacus in different functional states, Zhurn. evolyuc. biohim. i fiziol. 2002. T. 38. No. 1. P. 36–44.

Fingerman M., Lago A. D. Endogenous twenty four hour rhythms of locomotor activity and oxygen consumption in the crayfi sh Orconectes clypeatus, The American Midland Naturalist. 1957. Vol. 58. P. 383–393.

Ghanawi J., Saoud I. P. Molting, reproductive biology, and hatchery management of red claw crayfish Cherax quadricarinatus (von Martens 1868), Aquaculture. 2012. Vol. 358–359. P. 183–195.

Holodkevich S. V. Bioelectronic monitoring of the toxicity level of natural and sewage water in the real time regime, Ekologicheskaya himiya. 2007. T. 16. No. 4. P. 223–232.

Holodkevich S. V. Fiber-optic remote biosensor systems for permanent biological monitoring of the surface water and bottom sediments quality in the real time, Neft' i gaz arkticheskogo shel'fa – 2006: Materialy mezhdunarodnoy konferencii, Murmansk, 15–17 noyabrya. Murmansk: MMBI KNC RAN, 2006. P. 287–296.

Ivanov A. V. Holodkevich S. V. Kurakin A. S. Self-diagnostics of bioelectronics systems of environment monitoring in real time, Sistemy kontrolya okruzhayuschey sredy. 2012. No. 17. P. 26–31.

Kholodkevich S. V., Ivanov A. V., Kurakin A. S., Kornienko E. L., Fedotov V. P. Real time biomonitoring of surface water toxicity level at water supply stations, J. Environmental Bioindicators. 2008. Vol. 3. No. 1. P. 23–34.

Kozák P., Kuklina I. Crayfish as tools of water quality monitoring, Freshwater Crayfish: A Global Overview, CRS Press, eds. T. Kawai, Z. Faulkes, G. Scholtz. Boca Raton, 2016. P. 275–298.

Kuznecova T. V. Sladkova S. V. Holodkevich S. V. Evaluation of functional state of crayfish in normal and toxic environment by characteristics of their cardiac activity and hemolymph biochemical parameters, Zhurn. evol. biohim. i fiziol. 2010. T. 46. No. 3. P. 203–210.

Mel'nik E. A. Rublevskaya O. N. Pankova G. A. Holodkevich S. V. Ivanov A. V. Kornienko E. L. Sladkova S. V. Lyubimcev V. A. Kurakin A. S. Bioelectronic system for toxicological safety control of biologically purified wastewater, Vodosnabzhenie i sanitarnaya tehnika. 2013. No. 1. C. 7–12.

Nakayama S. M., Ikenaka Y., Muzandu K,. Choongo K., Oroszlany B., Teraoka H., Mizuno N., Ishizuka M. Heavy metal accumulation in lake sediments, fish (Oreochromis niloticus and Serranochromis thumbergi), and crayfish (Cherax quadricarinatus) in Lake Itezhi-tezhi and Lake Kariba, Zambia, Arch. Environ. Contam. Toxicol. 2010. Vol. 59. No. 2. P. 291–300.

Passano L. M. Molting and its control, Physiology of Crustacea. 1960. Vol. 1. P. 473–536.

Pollard T. G., Larimer J. L. Circadian rhythmicity of heart rate in the crayfish, Procambarus clarcii, Comp. Biochem. Physiol. 1977. Vol. 57(A). P. 221–226.

Reynolds J., Souty-Grosset C. Management of Freshwater Biodiversity. Cambridge: University press, 2012. 374 p.

Rice P. R., Armitage K. B. The effect of photoperiods on oxygen consumption of the crayfish Orconectes nais (Faxon), Comp. Biochem. Physiol. 1974. Vol. 47(A). P. 261–270.

Shechter A., Berman A., Singer A., Freiman A., Grinstein M., Erez J., Aflalo E. D., Sagi A. Reciprocal Changes in Calcification of the Gastrolith and Cuticle During the Molt Cycle of the Red Claw Crayfish Cherax quadricarinatus, Biol. Bull. 2008. Vol. 214. P. 122–134.

Sladkova C. V. Safronova D. V. Holodkevich S. V. The study of the effect of light intensity, temperature and feeding conditions changes on the cardiac activity of crayfish-bioindicators in bioelectronics systems for surface water quality monitoring, Vestnik Sankt-Peterburgskogo universiteta. Seriya 3. Biologiya. 2016. No. 1. P. 137–149.

Sladkova S. V. Holodkevich S. V. Total protein in hemolymph of crayfish Pontastacus leptodactylus as a parameter of the functional state of animals and a biomarker of habitat quality, Zhurn. evol. biohim. i fiziol. 2011. T. 47. No. 2. P. 136–141.

Styrishave B., Bojsen B. N., Witthofft H., Andersen O. Diurnal variations in physiology and behaviour of the noble crayfish Astacus astacus and the signal crayfish Pacifastacus leniusculus, Marine and Freshwater Behaviour and Physiology. 2007. Vol. 40. No. 1. P. 63–77.

Styrishave B., Rasmussen A. D., Depledge M. H. The influence of bulk and trace metals on the circzdian rhythm of heart rates in freshwater crayfish Astacus astacus, Marine Pollution Bulleten. 1995. Vol. 31. No. 1–3. P. 87–92.

Udalova G. P. Holodkevich S. V. Sladkova S. V. Ivanov A. V. Rymsha V. A. Study of circadian activity in the crayfish Pontastacus leptodactylus during their multimonth maintenance in the river water flow, Zhurn. evolyuc. biohim. i fiziol. 2009. T. 45. No. 3. P. 304–312.

Westin L., Gydemo R. Influence of light and temperature on reproduction and moulting frequency of the crayfish, Astacus astacus L., Aquaculture. 1986. Vol. 52. P. 43–50.

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