|Issue № 2||
Methods of ecological investigations
|Ph.D., Saint-Petersburg Scientific Research Center of Ecological Safety, Russian Academy of Sciences, email@example.com|
|Dr.Sci.Tech, Institute of Earth Sciences, Saint-Petersburg State University, firstname.lastname@example.org|
Summary: Original fiber-optic noninvasive method for registration and analysis of cardiac rhythm in macrobenthal invertebrates with shells was elaborated in the 1990s. This method is based on infrared light remote registration of the heartbeat change. The aim of this study is to adapt this methodological approach for freshwater bivalves. We estimated heart rate in the three species of freshwater bivalves (Unio pictorum, Anodonta anatine and Dreissena polymorpha) collected from the Neva River estuary. The Spearman rank correlations found significant (p < 0.05) relationships between the heart rate of bivalves, water temperature and shell size. The heart rate of Anodonta correlates positively (R = 0.92, p = 0.00) with water temperature and negatively (R = – 0.90, p = 0.00) with the shell size. The rise of NaCl salt content in water up to 3 g/l was accompanied by the increase of heart rate in bivalves, and concentrations more than 5 g/l resulted in decrease of that. After the addition of 3-8 g/l of NaCl reliable data of heart rate changes were not found at water temperature less than 7 ºC.
© Petrozavodsk State University
Received on: 12 February 2015
Published on: 09 June 2015
Bahmet I. N. Berger V. Ya. Halaman V. V. Heart rate of mussel Mytilus edulis (Bivalvia) under change of salinity. Biologiya morya. 2005. No. 31(5). P. 363–366.
Zotin A. A. Vladimirova I. G. The respiration rate and life expectancy of the species of freshwater bivalves families Margaritiferidae and Unionidae, Izvestiya RAN. Seriya biologicheskaya. 2001. No. 3. P. 331–338.
Konstantinov A. S. General Hydrobiology. M.: Vyssh. shk., 1986. 472 p.
Martem'yanov V. I. Influence of the mineral composition of the external environment on the performance of water-salt metabolism of zebra mussel Dreissena polymorpha Pallas installed in Rybinsk Reservoir, Rossiyskiy zhurnal biologicheskih invaziy. 2011. T. 4. No. 2. P. 120–134.
Fedotov V. P. Holodkevich S. V. Strochilo A. G. The study of the contractile activity of the heart of crawfish using a new non-invasive method, Zhurnal evolyucionnoy biohimii i fiziologii. 2000. T. 36, No. 3. P. 219–222.
Holodkevich S. V. Shumilova T. E. Fedotov V. P. Sladkova S. V. Express method for assessment of functional state of the crayfish as a tool to test the sustainability of ecosystems of small reservoirs, Trudy IV Vserossiyskoy nauchno-prakticheskoy konferencii s mezhdunarodnym uchastiem «Novoe v ekologii i bezopasnosti zhiznedeyatel'nosti» 16–18 iyunya 1999 goda. SPb., 1999. T. 3. P. 451.
Holodkevich S. V. Ivanov A. V. Kornienko E. L. Kurakin A. S. Method for monitoring biological environment (variants) and the system for its realization, Byul. izobr. 2007. No. 29. Patent RF No. 2308720 C1, MPK G01N 33/18 (2006.01); G01N 21/17 (2006.01).
Shkorbatov G. L. Starobogatov Ya. I. Methods of studying bivalve molluscs, Trudy Zoologicheskogo instituta AN SSSR. T. 219. L., 1990. 208 p.
Bakhmet I. N., Zdorovenov P. E. Variability in cardiac activity of the bivalves Mytilus edulis and Modiolus modiolus, Russian Journal of Marine Biology. 2010. Vol. 36, No. 3. P. 223–225.
Braby C. E., Somero G. N. Following the heart: temperature and salinity effects on heart rate in native and invasive species of blue mussels (genus Mytilus), J. Exp. Biol. 2006. Vol. 20. R. 2554–2566.
Burnett N. P., Seabra R., Pirro M., Wethey D. S., Woodin S. A., Helmuth B., Zippay M. L., Sarà G., Monaco C., Lima F. P. An improved noninvasive method for measuring heartbeat of intertidal animals, Limnol. Oceanogr. Methods. 2013. No. 11. P. 91–100.
Clausen I., Riisgard H. U. Growth, filtration and respiration in the mussel Mytilus edulis: no evidence for physiological regulation of the filter-pump to nutritional needs, Mar. Ecol. Progr. Ser. 1996. Vol. 141. P. 37–45.
Depledge M. H., Andersen B. B. A computer-aided physiological monitoring system for continuous, long-term recording of cardiac activity in selected invertebrates, Comp. Biochem. Physiol. 1990. Vol. 96. R. 474–477.
Depledge M. H., Lundebye A. K., Curtis T., Aagaard A., Andersen B. B. Automated interpulse-duration assessment (AIDA): a new technique for detecting disturbances in cardiac activity in selected invertebrates, Mar. Biol. 1996. Vol. 126, No. 2. P. 313–319.
Kholodkevich S. V., Ivanov A. V., Kurakin A. S., Kornienko E. L., Fedotov V. P. Real time biomonitoring of surface water toxicity level at water supply stations, Journal of Environmental Bioindicators. 2008. Vol. 3, No. 1. P. 23–34.
Kholodkevich S. V., Ivanov A. V., Kornienko E. L., Kurakin A. S. Method of biological environment monitoring (versions) and a system for realization thereof. 05.14.2013. US Patent NO. 8442809.
Burnett N. P. , Seabra R. , de Pirro M., Wethey D. S., Woodin S. A., Helmuth B., Zippay M. L. , Sarà G. , Monaco C., Lima F. P. An improved noninvasive method for measuring heartbeat of intertidal animals., Limnol. Oceanogr. Methods. 2013. Vol. 11. P. 91-100.