| Issue № 2 |
Original research |
pdf-version |
Assessment of morphological features and antioxidant activity of radish (Raphanus sativus) in conditions of soil pollution by heavy metals and petroleum products
| Sindireva Anna Vladimirovna | D.Sc., Associate Professor, Federal State Autonomous Educational Institution of Higher Education Tyumen State University, 625003 Russia Tyumen Volodarsky St. 6, sindireva72@mail.ru |
| Zaostrovskaya Veronika Vladimirovna | Federal State Autonomous Educational Institution of Higher Education Tyumen State University, 625003 Russia Tyumen Volodarsky St. 6, stud0000212862@utmn.ru |
| Veshkurtseva Svetlana Semyonovna | Federal State Autonomous Educational Institution of Higher Education Tyumen State University, 625003 Russia Tyumen Volodarsky St. 6, s.s.veshkurceva@utmn.ru |
|
Keywords: heavy metals petroleum products radish photosynthetic pigments oxidative stress antioxidant activity |
Summary: In phytotesting polluted soils, the indicators of plant growth and development serve as an integral assessment. At the same time, biochemical markers are of particular interest, among which products of oxidative stress and antioxidant substances are of particular importance. The article evaluates the morphological changes and antioxidant activity of a plant organism using the example of a test culture of radish (Raphanus sativus) in conditions of soil contamination with heavy metals and petroleum products. Five series of experiments were conducted using the seedling method according to the following scheme: 1) control – soil; 2) experimental samples: the soil with the addition of acetate salts of copper (in doses from 27.5 to 110 mg/kg), zinc (11.5–46 mg/kg), lead (15–60 mg/kg), cadmium (0.25–1 mg/kg), as well as petroleum products (100–1000 mg/kg). The laboratory germination of seeds, growth and development indicators of radish seeds under the influence of heavy metals and petroleum products were studied. The addition of zinc (in doses from 12.5 to 46 mg/kg) and lead (in doses from 15 to 60 mg/kg) contributed to an increase in laboratory germination of radish seeds. Copper had no significant effect on the initial growth and development of radishes. Cadmium and petroleum products in the studied doses reduce the laboratory germination of radish seeds. The effect of ecotoxicants on the content of photosynthetic pigment components, the amount of phenolic and flavonoid compounds was revealed. The content of lipid peroxidation products (diene conjugates and Schiff bases) in plants under conditions of soil contamination with Cd, Pb, Zn, Cu and petroleum products was estimated. The state of antioxidant activity in plants in response to stress caused by additional intake of ecotoxicants into the soil was determined. The level of pro-oxidant and antioxidant processes in the plant body depends on the dose and chemical properties of the introduced heavy metals and petroleum products. © Petrozavodsk State University |
Received on: 10 February 2025 Published on: 28 May 2025 | |
References
Anjum N. A. Sofo A. Scopa A. et al.Lipids and proteins – major targets of oxidative modifications in abiotic stressed plants , Environmental Science and Pollution Research. 2015. No. 22. P. 4099–4121.
Chinmayee M. Anu M. S. Mahesh B. et al.A comparative study of heavy metal accumulation and antioxidant responses in Jatropha curcas L. , Journal of Environmental Science, Toxicology and Food Technology. 2014. Vol. 8, No. 7. P. 58–67.
Emamverdian A., Ding Y., Mokhberdoran F. Review article: Heavy metals and some mechanisms of plant defense response, The Scientific World Journal. 2015. No. 4. P. 1–18.
Erofeeva E. A. Hormesis and paradoxical effects in plants under conditions of automobile pollution and under the action of pollutants in an experiment: Dip. ... kand. biol. nauk. N. Novgorod, 2016. 45 p.
Fedorova A. I. Nikol'skaya A. N. Practicum on ecology and environmental protection. M.: VLADOS, 2001. P. 101–104.
Lovineckaya S. B. Eremeeva V. G. Sindireva A. V. Assessment of the content of petroleum products in the soils of the roadside territories of Omsk and the Omsk region and the possibility of their remediation, Omskiy nauchnyy vestnik. 2015. No. 1 (138). P. 241–245.
Nikitina V. S. Shendel' G. V. Orazov O. E. Plant phenolic compounds as indicators of industrial environmental pollution, Aktual'nye problemy ekologii. 2005. No. 2. P. 124–127.
Osipova E. S. Petuhova G. A. Perekupka A. G. Activation of biochemical plant protection mechanisms under the influence of oil pollution and paraaminobenzoic acid, Vestnik Tyumenskogo gosudarstvennogo universiteta. 2013. No. 6. P. 41–47.
Petuhov A. S. Hritohin N. A. Petuhova G. A. Lipid peroxidation in plant cells in an urban environment, Vestnik RUDN. Seriya: Ekologiya i bezopasnost' zhiznedeyatel'nosti. 2018. T. 26, No. 1. P. 82–90.
Pikovskiy Yu. I. Gennadiev A. N. Chernyanskiy S. S. Saharov G. N. The problem of diagnostics and normalization of soil pollution by oil and petroleum products, Pochvovedenie. 2003. No. 9. P. 1132–1140.
Shul'gin I. A. Nichipirovich A. A. Calculation of pigment content using nomograms, Hrolofill. Minsk: Nauka i tehnika, 1974. P. 127–136.
Shvedova A. A. Polyanskiy N. B. A method for determining the end products of lipid peroxidation in tissues-fluorescent Schiff bases, Issledovanie sinteticheskih i prirodnyh antioksidantov in vitro i in vivo: Sb. nauch. statey, Pod red. E. B. Burlakovoy. M.: Nauka, 1992. P. 72–73.
Sindireva A. V. Ecological and toxicological assessment of the effects of cadmium, zinc, selenium in the conditions of the southern forest-steppe of the Omsk region, Vestnik KrasGAU. 2011. No. 10 (61). P. 118–122.
Tret'yakov N. N. Koshkin E. I. Makrushin N. M. Physiology and biochemistry of agricultural plants. M.: Kolos, 2000. 640 p.
© 2011 - 2025
Petrozavodsk State University
technical support - RCNIT
The editor-in-chief - Andrey Korosov
ecopri@petrsu.ru